Giraud Lab

Cochin Institute
INSERM U1016
Batiment Gustave Roussy 410
27 rue du Faubourg Saint Jacques
75014 Paris, France

Matthieu Giraud, PhD

Faculty
matthieu.giraud[at]inserm.fr tel: +33 1 4051 66 06

Our main research interest is to decipher the molecular mechanisms leading to the establishment of immune tolerance to self and therefore preventing autoimmune manifestations and diseases.
The AIRE protein has been identified to induce a broad spectrum of auto-antigens in the thymus whose processed peptides are presented to maturing T cells that differentiate and escape the thymus only if they are blind to the complete repertoire of peptides presented in the thymus.
The mode of action of AIRE is unique by the large number of genes that AIRE is able to induce and also by the fact that auto-antigens are significantly over-represented in its signature.
AIRE is capable to «wake up» silenced genes by impacting chromosomal regions bearing repressed chromatin marks.

People

Clotide Guyon, PhD student

Nada Jmari, Engineer

Yen-Chin Li, Bioinformatics Scientist

Marie-Agnès Blaton, Assistant Engineer

Funding

ANR «Chair of Excellence» Grant

The Giraud lab received the highly competitive Grant «Chair of Excellence» from the French government, therefore the lab will be expending over the next few years. [Jul 2011]

«Marie Curie» reintegration Grant

The Giraud lab received a «Marie Curie» european reintegration Grant from the European Commission. [Jun 2011]

Selected Pubications

An RNAi screen for Aire cofactors reveals a role for Hnrnpl in polymerase release and Aire-activated ectopic transcription

Giraud M, Jmari N, Du L, Carallis F, Nieland TJ, Perez-Campo FM, Bensaude O, Root DE, Hacohen N, Mathis D, Benoist C.
Proc Natl Acad Sci U S A. 2014 Jan 16

Aire unleashes stalled RNA polymerase to induce ectopic gene expression in thymic epithelial cells

Giraud M, Yoshida H, Abramson J, Rahl PB, Young RA, Mathis D, Benoist C.
Proc Natl Acad Sci U S A (2011) Dec 27. [Epub ahead of print]

Both polymorphic VNTR and AIRE modulate differential expression of insulin in human thymic epithelial cells

Cai CQ, Zhang T, Breslin MB, Giraud M, Lan MS
Diabetes (2011) 60, 336-44

Aire's partners in the molecular control of immunological tolerance

Abramson J, Giraud M, Benoist C, Mathis D
Cell (2010) 140, 123-35

An IRF8-binding promoter variant and AIRE control CHRNA1 promiscuous expression in thymus

Giraud M, Taubert R, Vandiedonck C, Ke X, Levi-Strauss M, Pagani F, Baralle FE, Eymard B, Tranchant C, Gajdos P, Vincent A, Willcox N, Beeson D, Kyewski B, Garchon HJ
Nature (2007) 448, 934-7